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2016 Lexus RX350
2016 Lexus RX350
V6 3.5L
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  • Guides
  • Lexus RX350
  • 2016
  • How to Replace the Thermostat on a 2016 Lexus RX350 3.5L V6 (Coolant Drain & Bleed)
Thermostat Replacement 3.5 Toyota Highlander/ Lexus RX350  2008 - 2016 p0128

Thermostat Replacement 3.5 Toyota Highlander/ Lexus RX350 2008 - 2016 p0128

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Tools & Fluids

3 Ton
3 Ton
Floor Jack
3 Ton
3 Ton
Jack Stands
Wheel Chocks
Wheel Chocks
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Safety
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How to Replace the Thermostat on a 2016 Lexus RX350 3.5L V6 (Coolant Drain & Bleed)

Step-by-step instructions with required tools/parts, thermostat housing bolt torque specs, and safety tips for 2016

How to Replace the Thermostat on a 2016 Lexus RX350 3.5L V6 (Coolant Drain & Bleed)

Step-by-step instructions with required tools/parts, thermostat housing bolt torque specs, and safety tips for 2016

Orion
Orion

Assumption: Your RX350 has the factory 3.5L V6 and the thermostat is in the water inlet housing where the lower radiator hose attaches.

🔧 RX350 - Thermostat Replacement

The thermostat controls engine warm-up and operating temperature. If it’s stuck open you may get low heat and poor fuel economy; stuck closed can cause overheating. This job involves draining coolant, removing the thermostat housing, replacing the thermostat/gasket, then refilling and bleeding air from the cooling system.

Difficulty Level: Intermediate | Estimated Time: 2-4 hours


⚠️ Safety & Precautions

  • ⚠️ Only work on a fully cold engine; hot coolant can cause severe burns.
  • ⚠️ Support the RX350 with jack stands; never rely on a floor jack alone.
  • ⚠️ Keep coolant off belts and electrical connectors; clean spills immediately.
  • ⚠️ Coolant is toxic to pets/people; drain into a sealed container and dispose properly.
  • 🔋 Battery disconnect is not required, but keep hands/tools clear of the radiator fans (they can turn on automatically).

🔧 Required Tools

You'll need the following tools for this repair:

  • Floor jack (rated 3-ton minimum)
  • Jack stands (rated 3-ton minimum)
  • Wheel chocks
  • Safety glasses
  • Nitrile gloves
  • Drain pan (at least 3-gallon)
  • Spill-free coolant funnel kit
  • 10mm socket
  • 12mm socket
  • 3/8" drive ratchet
  • 3" extension (3/8" drive)
  • Torque wrench (5–60 Nm range)
  • Hose clamp pliers
  • Pick tool
  • Trim clip tool
  • Flathead screwdriver
  • Shop towels
  • Flashlight

🔩 Required Parts

HowToo sells all the parts you need for this repair:

  • Engine thermostat - Qty: 1
  • Thermostat gasket / O-ring - Qty: 1
  • Toyota / Lexus Super Long Life Coolant (pink, premixed) - Qty: 2 gallons
  • Lower radiator hose clamp - Qty: 1

📋 Before You Begin

  • 🅿️ Park on level ground, set the parking brake, and chock the rear wheels.
  • 🌡️ Make sure the engine is completely cold (ideally sit overnight).
  • 📦 Lay cardboard under the front of the RX350 to catch drips.
  • 🧰 Know your tools: a torque wrench is a tool that tightens bolts to an exact spec so you don’t strip or crack parts.

🔨 Step-by-Step Instructions

Follow these steps in order:

Step 1: Raise the front safely

  • Use floor jack to lift the front at the proper center jack point.
  • Set the RX350 onto jack stands and keep wheel chocks in place.

Step 2: Remove the lower engine cover (if equipped)

  • Use a trim clip tool to pop plastic clips.
  • Use a 10mm socket and ratchet to remove the bolts.
  • Keep clips/bolts grouped in a tray.

Step 3: Drain the coolant

  • Place a drain pan (at least 3-gallon) under the radiator drain area.
  • Slowly remove the radiator cap with a shop towel (turn to first stop, then off).
  • Open the radiator drain cock using a flathead screwdriver if needed (some are hand-turn style).
  • Let coolant drain fully into the drain pan.

Step 4: Remove the air intake duct for access

  • Use a flathead screwdriver to loosen the hose clamps on the intake duct.
  • Use a 10mm socket and ratchet to remove any duct mounting bolts.
  • Lift the duct out and set it aside.

Step 5: Locate the thermostat housing

  • Use a flashlight to find the lower radiator hose.
  • Follow it to the engine; it attaches to the thermostat (water inlet) housing.

Step 6: Remove the lower radiator hose from the housing

  • Use hose clamp pliers to compress the clamp and slide it back on the hose.
  • Twist the hose gently by hand to break it free.
  • If it’s stuck, use a pick tool carefully around the hose end to release it (don’t gouge the plastic/metal nipple).
  • Pull the hose off and aim it into the drain pan for remaining coolant.

Step 7: Remove the thermostat housing

  • Use a 10mm socket (sometimes 12mm socket) with a ratchet and extension to remove the housing bolts.
  • Pull the housing straight off. Expect some coolant to spill—use shop towels.

Step 8: Replace the thermostat and gasket

  • Remove the old thermostat and the old gasket / O-ring by hand.
  • Clean the mating surfaces using shop towels. Do not scratch the sealing surface.
  • Install the new thermostat in the same orientation as the old one.
  • Install the new gasket / O-ring (do not reuse the old one).
  • Match orientation before tightening anything.

Step 9: Reinstall the housing and torque bolts

  • Reinstall the housing and hand-start all bolts.
  • Use a torque wrench (5–60 Nm range) to tighten evenly.
  • Torque to 10 Nm (89 in-lbs).

Step 10: Reinstall the lower radiator hose

  • Push the hose fully onto the housing nipple by hand.
  • Use hose clamp pliers to position the clamp back in its original spot.
  • If the clamp feels weak or distorted, replace it with the new one.

Step 11: Reinstall the air intake duct and covers

  • Reinstall the intake duct and tighten clamps using a flathead screwdriver.
  • Reinstall the lower engine cover using a 10mm socket, ratchet, and trim clip tool.

Step 12: Refill and bleed air from the cooling system

  • Make sure the radiator drain cock is closed (snug, not over-tight).
  • Install a spill-free coolant funnel kit at the radiator fill neck.
  • Slowly add Toyota / Lexus Super Long Life Coolant (pink, premixed) until full.
  • Start the engine and let it idle. Set cabin heat to MAX temperature (highest heat) so coolant flows through the heater core.
  • As the engine warms up, watch for air bubbles in the funnel and keep topping off.
  • When the thermostat opens, the upper hose will get hot and the coolant level may drop—top off again.
  • Once bubbles stop and the cooling fans cycle, shut the engine off and let it cool.
  • Top off the radiator and then set the overflow reservoir to the FULL line.

✅ After Repair

  • 🔍 Check for leaks around the thermostat housing and lower hose with the engine running (use a flashlight).
  • 🌡️ Watch the temperature gauge on a 10–15 minute drive; it should warm up normally and stay stable.
  • 🧪 After the engine fully cools, recheck coolant level in the reservoir and top off if needed.
  • 🧼 Rinse any spilled coolant off the underbody area using water and wipe dry.

💰 DIY vs Shop Cost

Shop Cost: $450-$850 (parts + labor)

DIY Cost: $60-$180 (parts only)

You Save: $270-$790 by doing it yourself!

Shop labor rates vary but typically run $100-$150/hour. This repair takes a shop approximately 2-3 hours.


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