How to Replace the Thermostat on a 2016-2021 Lexus RX350 (Cooling System DIY Guide) (Engine: V6 3.5L)
Step-by-step instructions with required tools, parts list, coolant refill/bleed tips, and thermostat housing torque specs
How to Replace the Thermostat on a 2016-2021 Lexus RX350 (Cooling System DIY Guide) (Engine: V6 3.5L)
Step-by-step instructions with required tools, parts list, coolant refill/bleed tips, and thermostat housing torque specs for 2016, 2017, 2018, 2019, 2020, 2021
🔧 RX - Thermostat Replacement
The thermostat controls coolant flow to help your RX warm up quickly and then stay at the correct operating temperature. Replacing it involves draining some coolant, removing the thermostat housing, installing the new thermostat and seal, then refilling and bleeding air out of the cooling system.
Difficulty Level: Intermediate | Estimated Time: 2-4 hours
⚠️ Safety & Precautions
- ⚠️ Never open the cooling system when hot; wait until the engine is fully cold.
- ⚠️ Coolant is toxic—keep it away from kids/pets and clean spills immediately.
- ⚠️ Support the RX with jack stands before working underneath; never rely on a jack alone.
- ⚠️ Wear eye protection when draining coolant (it can splash).
- ⚠️ Battery disconnect is not required for this job, but keep the key fob away from the vehicle while hands are near moving parts.
🔧 Required Tools
You'll need the following tools for this repair:
- Floor jack (rated 3-ton minimum)
- Jack stands (rated 3-ton minimum)
- Wheel chocks
- Safety glasses
- Nitrile gloves
- Drain pan (10-quart minimum)
- Shop rags
- 10mm socket
- 12mm socket
- 3/8" drive ratchet
- 3/8" drive extension (6")
- Torque wrench (5-30 Nm range)
- Trim clip removal tool
- Hose clamp pliers
- Pick tool
- Funnel
- Spill-free coolant funnel kit (specialty)
🔩 Required Parts
HowToo sells all the parts you need for this repair:
- Engine thermostat - Qty: 1
- Thermostat seal / O-ring (gasket) - Qty: 1
- Toyota / Lexus Super Long Life Coolant (pink, pre-mixed) - Qty: 2 gallons
- Hose clamp (optional, if original is weak) - Qty: 1
📋 Before You Begin
- Park on level ground, set the parking brake, and install wheel chocks.
- Let the engine cool completely (best: sit overnight).
- Set the cabin heat to HI (this helps coolant flow through the heater core during bleeding).
- Place a drain pan (10-quart minimum) under the front of the RX.
- Hose clamp pliers are pliers that lock clamps open.
- A spill-free funnel kit seals to the radiator fill neck.
🔨 Step-by-Step Instructions
Follow these steps in order:
Step 1: Raise and support the RX
- Use a floor jack (rated 3-ton minimum) to lift the front of the RX at the front center jacking point.
- Place jack stands (rated 3-ton minimum) under the proper support points and lower the RX onto them.
- Give the vehicle a gentle shake to confirm it’s stable.
Step 2: Remove the lower engine cover (undertray)
- Use a 10mm socket and 3/8" drive ratchet to remove the undertray bolts.
- Use a trim clip removal tool to pop out any plastic clips without breaking them.
- Set all fasteners aside in a small pile so nothing gets lost.
Step 3: Drain coolant from the radiator
- Position the drain pan (10-quart minimum) under the radiator drain area.
- Slowly loosen the radiator drain cock (petcock) and let coolant drain into the pan.
- Use shop rags to control splashes.
- Once flow slows, close the drain cock snugly by hand (do not over-tighten).
Step 4: Remove the engine appearance cover and intake ducting (as needed for access)
- Remove the top engine cover by pulling upward firmly (it’s press-fit).
- If the intake duct blocks access, use a 10mm socket and 3/8" drive ratchet to loosen clamps/bolts and move the duct aside.
- Take a quick phone photo before moving hoses.
Step 5: Locate the thermostat housing
- Follow the lower radiator hose to the engine; the thermostat sits behind the hose connection at the thermostat/water inlet housing.
- Use a flashlight (phone light is fine) to identify the housing bolts and hose clamp.
Step 6: Remove the lower radiator hose from the thermostat housing
- Use hose clamp pliers to compress the spring clamp and slide it back on the hose.
- Twist the hose gently by hand to break it loose, then pull it off.
- Use a pick tool carefully around the hose end if it’s stuck (don’t gouge the plastic/metal neck).
- Expect some coolant to spill—keep the drain pan underneath.
Step 7: Remove the thermostat housing
- Use a 10mm socket, 3/8" drive ratchet, and 3/8" drive extension (6") to remove the housing bolts.
- Pull the housing straight off; a little coolant may drain out.
- Note the thermostat orientation before removal.
Step 8: Replace the thermostat and seal
- Remove the old thermostat and old thermostat seal / O-ring.
- Clean the sealing surfaces with shop rags (no scratches, no leftover gasket material).
- Install the new thermostat in the same orientation as the old one.
- Install the new thermostat seal / O-ring (make sure it sits flat and isn’t twisted).
Step 9: Reinstall the housing and torque the bolts
- Reinstall the housing by hand-starting all bolts to avoid cross-threading.
- Use a torque wrench (5-30 Nm range) and 10mm socket to tighten evenly.
- Torque to 10 Nm (89 in-lbs)
- Snug first, then final torque—prevents leaks.
Step 10: Reinstall the hose and clamp
- Slide the hose back onto the thermostat neck fully.
- Use hose clamp pliers to move the clamp back to its original position.
- Wipe the area clean with shop rags so any future leak is easy to spot.
Step 11: Reinstall intake parts and the engine cover
- Reinstall any intake ducting using a 10mm socket and 3/8" drive ratchet.
- Press the engine cover back on until it snaps into place.
Step 12: Refill coolant
- Lower the RX off the jack stands using the floor jack (refilling/bleeding is easier on level ground).
- Install a spill-free coolant funnel kit (specialty) (or use a funnel) at the radiator fill neck.
- Slowly add Toyota / Lexus Super Long Life Coolant (pink, pre-mixed) until full.
- Fill the coolant reservoir to the FULL line.
Step 13: Bleed air from the cooling system
- Start the engine and let it idle.
- Keep the heater on HI and fan on medium.
- Watch the coolant level in the funnel and add coolant as the level drops.
- Use nitrile gloves and gently squeeze the upper and lower radiator hoses to help air move out.
- When the engine warms up, the thermostat should open and you’ll see a more steady flow; top off as needed.
- Once no more bubbles appear for a few minutes, shut the engine off and let it cool fully.
- After cooling, remove the funnel and install the radiator cap securely.
Step 14: Reinstall the lower engine cover
- Reinstall the undertray using the 10mm socket, 3/8" drive ratchet, and trim clip removal tool.
- Make sure all clips and bolts are snug and the cover isn’t hanging down.
✅ After Repair
- Start the engine and check for leaks around the thermostat housing and hose connection.
- Take a 10-15 minute test drive, then recheck for leaks.
- After the engine fully cools (later that day or next morning), recheck the reservoir level and top off to the FULL line.
- If the temperature gauge rises abnormally or heat stops working, stop and re-bleed (air is still trapped).
💰 DIY vs Shop Cost
Shop Cost: $450-$850 (parts + labor)
DIY Cost: $60-$160 (parts only)
You Save: $290-$790 by doing it yourself!
Shop labor rates vary but typically run $100-$150/hour. This repair takes a shop approximately 2-4 hours.
🎯 Ready to get started?
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Guide for Engine Coolant Thermostat replace for these Lexus vehicles
| Year Make Model | Sub Model | Engine | Body Style |
|---|---|---|---|
| 2021 Lexus RX350 | - | V6 3.5L | - |
| 2020 Lexus RX350 | - | V6 3.5L | - |
| 2019 Lexus RX350 | - | V6 3.5L | - |
| 2018 Lexus RX350 | - | V6 3.5L | - |
| 2017 Lexus RX350 | - | V6 3.5L | - |
| 2016 Lexus RX350 | - | V6 3.5L | - |


















