Howtoo Logo
2014 Toyota Highlander
2001 - 2007 Toyota Highlander
Inline 4 2.4L
Compatible with more variants.
Bryan specialist avatar

Have a Question? Ask a Specialist

Here is everything needed for this repair

See what I can do

Make Money

With HowToo

OnOff

Here is just the beginning of what I can do!

Select one to see me in action

Vehicle Features

Image Vehicle Features

ā€œHow do I connect my phone to my stereo?ā€

Vehicle Information

Image Vehicle Information

ā€œWhat is my horsepower and torqueā€

Image Recognition

Image Image Recognition

ā€œWhat is this warning light on my dash?ā€

Troubleshooting

Image Troubleshooting

ā€œI have a P0300 engine codeā€

Vehicle Recognition

Image Vehicle Recognition

ā€œWhat vehicle is this?ā€

Find shops near you

Image Find shops near you

ā€œFind a shop to do this repairā€

Vehicle Talk

Image Vehicle Talk

ā€œWhat’s your favorite vehicle of all time?ā€

Thermostat Replacement 3.5 Toyota Highlander/ Lexus RX350  2008 - 2016 p0128

Thermostat Replacement 3.5 Toyota Highlander/ Lexus RX350 2008 - 2016 p0128

Suggested Parts

See all parts background
See All Parts

Tools & Fluids

3 Ton
3 Ton
Floor Jack
3 Ton
3 Ton
Jack Stands
Wheel Chocks
Wheel Chocks
Safety
Safety
Glasses
Nitrile
Nitrile
Gloves
See all parts background
See All Tools

How to Replace the Thermostat on a 2001-2016 Toyota Highlander (Cooling System Guide) (Engine: Inline 4 2.7L)

Step-by-step thermostat housing removal, coolant drain/refill, air bleeding, tools, parts, and torque specs

How to Replace the Thermostat on a 2001-2016 Toyota Highlander (Cooling System Guide) (Engine: Inline 4 2.7L)

Step-by-step thermostat housing removal, coolant drain/refill, air bleeding, tools, parts, and torque specs for 2001, 2002, 2003, 2004, 2005, 2006, 2007

Orion
Orion

šŸ”§ Highlander - Thermostat Replacement

The thermostat controls coolant flow to help your engine warm up quickly and prevent overheating. On your Highlander, it’s mounted in the thermostat housing on the front/side of the V6, and replacing it usually means draining some coolant, swapping the thermostat, then refilling and bleeding air.

Difficulty Level: Intermediate | Estimated Time: 2-4 hours


āš ļø Safety & Precautions

  • āš ļø Never open the cooling system hot; hot coolant can spray and burn.
  • āš ļø Support the Highlander with jack stands on solid, level ground; never rely on a jack alone.
  • āš ļø Keep coolant away from kids/pets; it’s poisonous and spills are slippery.
  • āš ļø Wear gloves and safety glasses; coolant can irritate skin and eyes.
  • āš ļø Battery disconnect is not required for this job, but keep hands/tools clear of the radiator fans (they can turn on).

šŸ”§ Required Tools

You'll need the following tools for this repair:

  • Floor jack (rated 3-ton minimum)
  • Jack stands (rated 3-ton minimum)
  • Wheel chocks
  • Safety glasses
  • Nitrile gloves
  • Drain pan (at least 10-quart)
  • Shop towels
  • 10mm socket
  • 3/8" drive ratchet
  • 6" extension (3/8" drive)
  • Torque wrench (in-lb or low Nm range)
  • Hose clamp pliers
  • Pick tool
  • Plastic trim clip tool
  • Phillips screwdriver
  • Spill-free coolant funnel kit (specialty)

šŸ”© Required Parts

HowToo sells all the parts you need for this repair:

  • Engine thermostat - Qty: 1
  • Thermostat gasket / O-ring - Qty: 1
  • Toyota Super Long Life Coolant (pink, 50/50 premix) - Qty: 2-3 gallons
  • Replacement hose clamps - Qty: 2

šŸ“‹ Before You Begin

  • Park on level ground, set the parking brake, and place wheel chocks behind the rear wheels.
  • Let the engine cool completely (ideally overnight) so there is zero pressure in the cooling system.
  • Set the cabin HVAC to HOT later during bleeding so coolant can flow through the heater core (the small radiator for the cabin heat).
  • Plan for spills; lay towels under the work area.

šŸ”Ø Step-by-Step Instructions

Follow these steps in order:

Step 1: Raise and support the front (if needed for access)

  • Use a floor jack (rated 3-ton minimum) to lift the front at the approved front jacking point.
  • Place jack stands (rated 3-ton minimum) under the front support points and lower the Highlander onto them.
  • Keep wheel chocks in place.

Step 2: Remove the lower engine cover/splash shield

  • Use a plastic trim clip tool to pop out the plastic clips.
  • Use a 10mm socket with a 3/8" drive ratchet to remove any 10mm bolts.
  • Set the cover and clips aside in a safe spot.

Step 3: Drain coolant to below thermostat level

  • Place a drain pan (at least 10-quart) under the radiator drain area.
  • Slowly loosen the coolant fill cap (radiator cap or reservoir cap) to relieve any leftover pressure.
  • Open the radiator drain (drain cock) and let coolant drain until the radiator level is low.
  • Use shop towels to wipe any spills immediately.

Step 4: Locate the thermostat housing

  • Find the lower radiator hose (the thicker hose going from the radiator to the engine).
  • Follow that hose to where it bolts to the engine—this bolted ā€œneckā€ is the thermostat housing.

Step 5: Remove the radiator hose from the thermostat housing

  • Use hose clamp pliers to squeeze the spring clamp and slide it back on the hose.
  • Twist the hose gently by hand to break it free, then pull it off the housing.
  • If it’s stuck, use a pick tool carefully to break the seal (don’t gouge the plastic/aluminum).
  • Expect some coolant to spill—keep the drain pan under it.

Step 6: Remove the thermostat housing bolts

  • Use a 10mm socket, 3/8" drive ratchet, and 6" extension (3/8" drive) to remove the housing bolts.
  • Pull the thermostat housing off gently. If it sticks, wiggle it—do not pry hard on sealing surfaces.

Step 7: Replace the thermostat and gasket

  • Remove the old thermostat and the old gasket/O-ring.
  • Clean the mating surfaces with shop towels (no chunks of old gasket should remain).
  • Install the new thermostat in the same orientation as the old one.
  • Install the new gasket/O-ring fully seated (no twisting or pinching).

Step 8: Reinstall the thermostat housing

  • Position the housing squarely and start all bolts by hand to avoid cross-threading.
  • Use a torque wrench (in-lb or low Nm range) to tighten evenly.
  • Torque to 10 Nm (89 in-lb)
  • Snug first, then final torque—prevents leaks.

Step 9: Reinstall the hose and clamp

  • Push the hose fully onto the housing until it bottoms out.
  • Use hose clamp pliers to move the clamp back to its original position.
  • If the clamp is weak or rusty, replace it with a new clamp for a better seal.

Step 10: Reinstall the splash shield

  • Reposition the cover and install fasteners.
  • Use a 10mm socket and 3/8" drive ratchet for bolts.
  • Use a plastic trim clip tool to reinstall push-clips without breaking them.

Step 11: Refill coolant

  • Mixing is not needed if you bought Toyota Super Long Life Coolant (pink, 50/50 premix).
  • Install a spill-free coolant funnel kit (specialty) (this is a sealed funnel that lets air burp out without spilling).
  • Slowly add coolant until the level stays up.

Step 12: Bleed air and verify thermostat operation

  • Start the engine and let it idle.
  • Set HVAC to HOT and fan to medium.
  • Watch the funnel level; add coolant as the level drops.
  • As the engine warms up, you should feel the upper radiator hose get hot when the thermostat opens.
  • Let the cooling fans cycle at least once, then shut the engine off and let it cool.

Step 13: Final level check

  • Once fully cool, remove the funnel and install the cap.
  • Top off the coolant reservoir to the FULL line if needed.
  • Check for leaks around the housing and hose connection using safety glasses and a flashlight.

āœ… After Repair

  • Warm the engine on a short drive, then re-check for leaks with the engine running.
  • After the next full cool-down (later that day or next morning), re-check the reservoir level and top off if needed.
  • Make sure cabin heat works normally; weak heat can mean trapped air.
  • If the temperature gauge rises above normal, stop driving and re-check coolant level/air bleeding.

šŸ’° DIY vs Shop Cost

Shop Cost: $350-$700 (parts + labor)

DIY Cost: $60-$180 (parts only)

You Save: $170-$640 by doing it yourself!

Shop labor rates vary but typically run $100-$150/hour. This repair takes a shop approximately 2-3 hours.


šŸŽÆ Ready to get started?

HowToo makes it easy: same-day/2-day shipping on every part, plus all the tools and specialty tools you need! Check out the parts and tools sections below to add everything to your cart.

Assumption: Torque and layout shown match the common V6 thermostat housing on this Highlander; if your housing uses different bolt sizes, use the same steps but torque to the spec for your housing fasteners.

Guide for Engine Coolant Thermostat replace for these Toyota vehicles

Year Make ModelSub ModelEngineBody Style
2016 Toyota Highlander-V6 3.5L-
2016 Toyota Highlander-Inline 4 2.7L-
2015 Toyota Highlander-V6 3.5L-
2015 Toyota Highlander-Inline 4 2.7L-
2014 Toyota Highlander-V6 3.5L-
2014 Toyota Highlander-Inline 4 2.7L-
2013 Toyota Highlander-V6 3.5L-
2013 Toyota Highlander-Inline 4 2.7L-
2012 Toyota Highlander-V6 3.5L-
2012 Toyota Highlander-Inline 4 2.7L-
2011 Toyota Highlander-V6 3.5L-
2011 Toyota Highlander-Inline 4 2.7L-
2010 Toyota Highlander-V6 3.3L-
2010 Toyota Highlander-V6 3.5L-
2010 Toyota Highlander-Inline 4 2.7L-
2009 Toyota Highlander-V6 3.3L-
2009 Toyota Highlander-V6 3.5L-
2009 Toyota Highlander-Inline 4 2.7L-
2008 Toyota Highlander-V6 3.3L-
2008 Toyota Highlander-V6 3.5L-
2007 Toyota Highlander-Inline 4 2.4L-
2007 Toyota Highlander-V6 3.3L-
2006 Toyota Highlander-Inline 4 2.4L-
2006 Toyota Highlander-V6 3.3L-
2005 Toyota Highlander-Inline 4 2.4L-
2005 Toyota Highlander-V6 3.3L-
2004 Toyota Highlander-Inline 4 2.4L-
2004 Toyota Highlander-V6 3.3L-
2003 Toyota Highlander-Inline 4 2.4L-
2003 Toyota Highlander-V6 3.0L-
2002 Toyota Highlander-Inline 4 2.4L-
2002 Toyota Highlander-V6 3.0L-
2001 Toyota Highlander-Inline 4 2.4L-
2001 Toyota Highlander-V6 3.0L-
Parts
Tools
2014 Toyota Highlander
Menu
Videos
Earn